Asymmetric Reduction by an NADH Model Compound with l-Prolinamide in the N1-Substituent |
| |
Authors: | Fumitoshi Hoshide Naomichi Banba Jun’ichi Oda Yuzo Inouye |
| |
Institution: | 1. Fumakilla Co., Ltd., Ohno-cho, Saeki-gun, Hiroshima, 739–04 Japan;2. Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611 Japan |
| |
Abstract: | An intracellular uricase from Bacillus fastidiosus with high catalytic capacity and strong resistance to xanthine was inactivated in water but could be essentially re-activated in solutions of high ionic strength. By polyacrylamide gel electrophoresis (PAGE), gradient PAGE, sodium-dodecyl-sulfate-PAGE, gel-filtration through Sephadex G200, and activity staining with peroxidase and its chromatogenic substrate, this homotetrameric uricase in water was found to dissociate into inactive homodimers that could form active homotetramers again in solutions of high ionic strength. Sensitivity to low ionic strength of solutions complicates formulation of this uricase as a drug and its elimination requires protein engineering. |
| |
Keywords: | uricase dialysis inactivation dissociation of homotetramer ionic strength |
|
|