Purification of Oat Debranching Enzyme and Occurrence of Inactive Debranching Enzyme in Cereals |
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Authors: | Jiro Yamada |
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Affiliation: | Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan |
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Abstract: | The interaction of some anthracycline antibiotics (adriamycin, daunomycin, aclacinomycin-A) with bacteriophage ?X174 was investigated. Adriamycin and daunomycin inactivated the infectivity of both free ?X174 phage and naked single-stranded ?X174 DNA without DNA strand scission, but aclacinomycin-A did not show this action. The phage inactivation reaction was reversibly inhibited by Superoxide dismutase, catalase or other oxygen radical scavengers. The inactivation of ?X174 by adriamycin and aclacinomycin-A was stimulated by the addition of Cu2+, while the ?X174 inactivation by daunomycin was inhibited by the addition of Cu2+. The ?X174 inactivation by adriamycin and aclacinomycin-A in the presence of Cu2+ was caused by degradation of DNA, and this inactivation reaction was inhibited irreversibly by oxygen radical scavengers. These results indicate that anthracycline antibiotics bind to ?X174 DNA in the form of free radicals and that during the auto-oxidation of these antibiotics in the presence of Cu2+, oxygen radicals were generated to cause the degradation of ?X174 DNA. |
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Keywords: | endoglucanase cellulase glycoside hydrolase family 45 Zygomycota Mucorales |
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