Derepressive Formation of l-Leucine-Pyruvate Transaminase under Nitrogen-Free Conditions in Gluconobacter suboxydans |
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Authors: | Takashi Tachki Kousaku Murata Masayasu Bunno Tatsurokuro Tochdcura |
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Institution: | Department of Food Science and Technology, Kyoto University, Kyoto 606, Japan |
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Abstract: | l-Leucine-pyruvate transaminase activity increased 6- to 20-fold in 3 hr when Gluconobacter suboxydans cells grown on yeast extract-medium were transferred to and incubated in a nitrogen-free medium. The increase in enzyme activity was influenced remarkably by the age and concentration of cells used. The phenomenon depended upon de novo synthesis of enzyme protein.The enzyme activity in cell-free extracts of cells incubated under a nitrogen-free condition decreased remarkably after heat treatment at 50°C (pH 6.0) or after freezing and thawing. The level of such enzyme inactivation was high in extracts of cells in the early stages of induction and low in later stages. |
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Keywords: | red tide concanavalin A glycocalyx phytoplankton |
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