A New Isolation Method of Plasmid Deoxyribonucleic Acid from Staphylococcus aureus Using a Lytic Enzyme of Achromobacter lyticus |
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| Authors: | Sueharu Horinouchi Takeshi Uozumi Teruhiko Beppu Kei Arima |
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| Affiliation: | Laboratory of Fermentation, Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo, Japan |
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| Abstract: | An enzymatic procedure for the differential determination of polyamines, spermine and spermidine, has been established using beef plasma amine oxidase. This method was specific for these polyamines and required only one reaction system. Small amounts of polyamines (10µm to 80 µm of spermine and 10 µm to 100 µm of spermidine) were assayed by solving two simultaneous equations obtained from the rate assay method and the end point assay method. The calculated values were in good agreement with those obtained by other method. |
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