Effect of Sclerin on Incorporation of Oleic Acid into Phospholipids and Activity of Phospholipase in Mitochondria |
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Authors: | Masanori Yamaguchi Yukio Satomura |
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Institution: | Faculty of Science, Osaka City University, Osaka |
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Abstract: | Sclerin (SCL) stimulated the oxidation and the incorporation into the phospholipids of Na-1-14C]-oleate in mitochondria isolated from rat liver, preventing the depression of the phosphorylating functions and protecting 2,4-dinitrophenol (DNP)-activated ATPase in mitochondria during incubation with oleate. Also, SCL markedly enhanced the activity of phospholipase to hydrolyze endogenous substrates in mitochondria. The increase in the activity was due to reconstruction of phospholipids through esterification of oleate in mitochondrial membrane, but not to the de novo enzyme synthesis. It was concluded that the level of endogenous phospholipase in mitochondria during incubation reflects the energy- dependent reacylation of the lysophospholipids produced by the action of phospholipase in mitochondrial membrane. |
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Keywords: | adsorption emulsification method mechanical stress methyl linoleate oxidation |
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