Reactivation of Inactivated d-Glucose Dehydrogenase of a Bacillus Species by Pyridine and Adenine Nucleotides |
| |
Authors: | Akira Yokota Ken-ichi Sasajima Masahiko Yoneda |
| |
Institution: | Microbiological Research Laboratories, Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan |
| |
Abstract: | d-Glucose dehydrogenase β-d-glucosc: NAD(P) oxidoreductase (EC 1.1.1.47)] was synthesized derepressively in a mutant of a Bacillus species which was isolated as an improved strain for d-ribose production. The enzyme was very unstable and inactivated during storage or column chromatography. The inactivation was prevented in the presence of NAD+, NADP+ or certain salts. The inactive enzyme was reactivated by the addition of NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP or certain salts. The molecular weights of the inactive and active form of the enzyme were estimated to be about 45,000 and 80,000, respectively, by Sephadex G–150 gel filtration. Thus, it seems that the enzyme activity is regulated by monomer-dimer interconversion of the enzyme molecule. |
| |
Keywords: | CD3 lymphoma quantitative proteomics selected reaction monitoring mass spectrometry |
|
|