Asymmetric Reduction of Iminium Salt with Chiral Dihydropyridines |
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| Authors: | Naomichi Baba Kazuyoshi Nishiyama Jun’ichi Oda Yuzo Inouye |
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| Affiliation: | Institute for Chemical Research, Kyoto University, Uji, Kyoto |
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| Abstract: | A gram positive bacterium (strain No. 109) isolated from soil as a producer of cyclodextrinase was identified as Bacillus coagulans. The cyclodextrinase from B. coagulans was purified to a homogeneous state by disc-electrophoresis after Streptomycin treatment, DEAE-Sephadex column chromatography, Ultrogel AcA44 gel filtration and hydroxyapatite column chromatography. The molecular weight of the enzyme was determined to be 6.2}104 by sodium dodecyl-sulfate gel electrophoresis. The isoelectric point of the enzyme was pH 5.0. The enzyme was most active at pH 6.2 and 50°C, and stable up to 45°C at pH 7.0 and in the range of pH 6.0 ~ 7.3 at 40°C on 2 hr incubation. This enzyme hydrolyzed linear maltooligosaccharides (such as maltotetraose (G4), maltopentaose (G5) and maltohexaose (G6)) and α-, β- and α-cyclodextrins (CDs) faster than maltotriose (G3) and short chain amylose ( | |
| Keywords: | Sal k 4 Salsola kali profilin cross-reactivity |
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