Fructose-2,6-bisphosphate Activates Pyrophosphate: d-Fructose 6-Phosphate 1-Phosphotransferase from Euglena gracilis |
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Authors: | Kazutaka Miyatake Toshiki Enomoto Shozaburo Kitaoka |
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Affiliation: | Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka 591, Japan |
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Abstract: | When hexavalent chromium (Cr6+) tolerant Pseudomonas ambigua G-l was cultivated in nutrient broth containing 150 ppm Cr6 +, the Cr6+ content of the broth rapidly decreased. The Cr6+ reducing enzyme found in a cell-free extract of P. ambigua G-l required NADH but not NADPH as a hydrogen donor for the reduction of Cr6 +. The specific activities of cell-free extracts of several Cr6+ sensitive mutants derived from P. ambigua G-l showed decreases to one fourth to one tenth of that of P. ambigua G-l. Glucose protected the Cr6+ reducing enzyme against inac-tivation on dialysis. |
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