The Assignment of the 13C-NMR Spectrum of Lysocellin and Its Biosynthesis |
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Authors: | Noboru ōtake Haruo Seto Mitsuo Koenuma |
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Institution: | Institute of Applied Microbiology, The University of Tokyo,Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | The complete 18C-NMR assignment of lysocellin sodium salt has been obtained based on the 13C-13C double labeling method and comparison with its retroaldol degradation product as well as a structurally related polyether antibiotic, lasalocid A.In parallel, the biosynthesis of lysocellin was investigated by feeding 13C-labeled precursors followed by analyzing the resulting labeling patterns of the 13C-NMR spectra; a pathway to the antibiotic molecule is suggested which proceeds through condensation of two butyrate, eight propionate and one acetate units.In addition, a conversion of butyrate to propionate during the metabolic process has been disclosed. |
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