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Sugar Composition of Cell Wall Polysaccharides of Suspension-cultured Tobacco Cells
Authors:Kimio Katō  Masao Noguchi
Affiliation:Central Research Institute, The Japan Tobacco and Salt Public Corporation, 6-2, Umegaoka, Midori-ku, Yokohama 227
Abstract:Neutral sugar composition of cell walls of suspension-cultured tobacco cells was examined with the advance of culture age by an anion-exchange chromatography. Isolated cell walls gave on hydrolysis the following sugars: 2% of l-rhamnose, 6% of d-mannose, 26% of l-arabinose, 13% of d-galactose, 8% of d-xylose and 47% of d-glucose as neutral sugars. Little changes in composition of cell wall polysaccharides were recognized with the advance of culture age. Sugar composition of the extra-cellular polysaccharides was similar to that of hemicellulose fraction from cell walls. Pectinic acid gave on hydrolysis 2-O-(α-d-galactopyranosyluronic acid)-l-rhamnose, d-galacturonic acid and its oligosaccharides.
Keywords:apoptosis  cytotoxic T lymphocyte (CTL)  (2R,3R,4S)-2,3-epoxy-4-hydroxy-5-hydroxymethyl-6-(1E)-propenyl-cyclohex-5-en-1-one (ECH)  Fas ligand  (2R,3R,4S)-2,3-epoxy-4-hydroxy-5-hydroxymethyl-cyclohex-5-en-1-one (RKTS-33)
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