Purification and Properties of Cellulases from an Alkalophilic Streptomyces Strain |
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| Authors: | Ryozo Nakai Sueharu Horinouchi Takeshi Uozumi Teruhiko Beppu |
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| Institution: | Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | An alkalophilic Streptomyces strain, KSM-9, producing extracellular cellulases was isolated from soil. Three kinds of cellulases that preferentially hydrolyzed carboxymethylcellulose (CMC) were purified from the strain and designated as CMCase I, II and III. The optimum pH of CMCase I (Mr, 32,000) is 8.5 while those of CMCase II (Mr, 32,500) and III (Mr, 92,000) are at around pH 6.0. CMCase I hydrolyzed CMC in a more random fashion than the other two enzymes. |
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