Subcellular Location and Some Properties of Isocitrate Dehydrogenase Isozymes in Euglena gracilis |
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Authors: | Yuji Oda Kazutaka Miyatake Yoshihisa Nakano Shozaburo Kitaoka |
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Affiliation: | Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka 591, Japan |
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Abstract: | Under thiamine-deficient, aerobic culture conditions, Yarrowia lipolytica was found to produce D-(+)-2-hydroxyglutaric acid extracellularly in amounts of about 5 mg per ml of the culture filtrate, together with pyruvic and 2-ketoglutaric acids, from glucose or glycerol in a chemically defined medium. Under similar conditions, however, only a small amount of the hydroxy acid was produced from odd- or even-numbered n-alkanes. |
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