A New Screening Procedure on Selective Inhibitors against SV40-Transformed Cells |
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| Authors: | Masanobu Munekata Akira Takatsuki Kazukiyo Onodera Gakuzo Tamura |
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| Institution: | 1. Laboratory of Microbiology, Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;2. Laboratory of Biological Chemistry, Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | A comparative study of selective cytotoxicity between non-transformed and SV40-transformed cells has been undertaken in combination with antitumor agents having different mechanisms of action. The selective in vitro cytotoxicity assay (N/T assay) measured the decrease in cell proliferation under the microscope. SV40-transformed cells had higher sensitivity than nontransformed cells against these antitumor agents at low concentration; but an apparent dissimilar pattern of sensitivity was observed in some antimetabolites, such as cytosine arabinoside, 6-mercaptopurine, 5-fluorouracil, and in some inhibitors of energy-transfer. The results can be interpreted in terms of a possible difference in cytotoxicity between non-transformed and SV40-transformed cell lines. The N/T assay may be considered a convenient system for evaluating selective cytotoxicity for in vitro–in vivo comparisons |
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