Construction of New Shuttle Vectors for Acetbacter |
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| Authors: | Masahiro Fukaya Hajime Okumura Hiroshi Masai Takeshi Uozumi Teruhiko Beppu |
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| Institution: | 1. Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd., Handa-shi, Aichi 475, Japan;2. Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo 113, Japan |
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| Abstract: | Shuttle vector pMV301 was constructed by ligation of pMV102 found in A. aceti subsp. xylinum NBI 1002 to E. coli plasmid pACYC177. It is 6.0 kb in size, has unique restriction sites suitable for insertion of a foreign DNA fragment and confers ampicillin resistance to the Acetobacter host. This vector transforms A. aceti subsp. aceti 10-80S1 and industrial vinegar producer A. aceti subsp. xylinum NBI 1002 as well as E. coli. Various chimeric plasmids were also constructed by ligation of pMV102 to E. coli plasmids to examine the expression of drug resistance genes. In addition to the ampicillin resistance gene, resistance genes for kanamycin, chloramphenicol and tetracycline derived from E. coli plasmids were expressed in Acetobacter. Most of the constructed shuttle vectors were stably maintained in Acetobacter. |
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