Amylostatins,Other Amylase Inhibitors Produced by Streptomyces diastaticus subsp. Amylostaticus No. 2476 |
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| Authors: | Ken-ichi Fukuhara Hidetsugu Murai Sawao Murao |
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| Institution: | Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai 591, Japan |
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| Abstract: | Amylase inhibitors (amylostatins) other than those reported as S-AI were found in the culture filtrate of Streptomyces diastaticus subsp. amylostaticus No. 2476. They were separated grossly into F-1a, F-1b and F-2 fractions by column chromatography on Dowex 50W × 4 (NH4+) and by preparative high performance liquid chromatography. Each fraction was further separated by preparative paper partition chromatography (PPC). Fractions obtained by PPC had different inhibitory activities against various amylases. On the other hand, acid hydrolysis of each active inhibitory fraction produced amylostatin X′ (C13H21NO7) and/or amylostatin XG (C19H33NO13). The diversities and common features of these amylostatins are discussed. |
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| Keywords: | aldose-ketose isomerase 5-methylthioribose-1-phosphate isomerase eukaryotic initiation factor 2B α-like protein methionine salvage pathway Bacillus subtilis |
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