Purification and Chemical Analysis of Microbial Cell Flocculant Produced by Aspergillus sojae AJ7002 |
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Authors: | Junji Nakamura Shigeyoshi Miyashiro Yoshio Hirose |
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Affiliation: | Central Research Laboratories of Ajinomoto Co., Inc., Kawasaki, Japan |
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Abstract: | A bio-flocculant was isolated from the culture broth of Asp. sojae AJ 7002. It was partially purified by acetone or ethanol precipitation, by ion-exchange and gel chromatography, and by dialysis. The isolated polymer possessed chemical characteristics of a poly-hexosamine and a protein. Glucosamine and galactosamine were not acetylated. The flocculant contained 2-ketogluconic acid, but sulfur or phosphorus was not detected. This flocculant was thermo-stable and its activity varied with pH. It was suggested that the hexosamine moiety in the polymer played a major role in bio-flocculation, assisted by protein portion in enlargement of the molecular weight of the flocculant, and by 2-ketogluconic acid in endowing it with amphoteric character. |
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Keywords: | crystal structure α-amylase TVA II pullulan |
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