Milk Fat Globule Membrane Proteins in Aseptically Packed Ultra-heat-treated Milk: Changes during Storage |
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Authors: | Kunio Yamauchi Makoto Shimizu Tsuyoshi Ando |
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Institution: | Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | Five genes for tryptophan biosynthesis, trpE, trpD, trpC, trpB, and trpA of Brevibacterium lactofermentum, a coryne form glutamic acid-producing bacterium, were cloned as a 9.6 kb BamHl DNA fragment by colony hybridization. A previously cloned 1.2 kb Pst I DNA fragment containing a major part of the trpE gene was used as a probe. By complementation tests using the subclones of this 9.6 kb BamHl fragment and various tryptophan auxotrophs of B. lactofermentum and Escherichia coli, this fragment was found to contain a gene cluster composed of trpE, trpD, trpC, trpB, and trpA in this order. It suggests that genes for tryptophan biosynthesis in B. lactofermentum may be an operon. |
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Keywords: | lysozyme lysozyme-catalyzed reaction 1H-NMR site-directed mutagenesis transglycosylation |
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