Staurosporine,a Prolyl Endopeptidase Inhibitor |
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| Authors: | Ken-ichi Kimura Noboru Kawaguchi Makoto Yoshihama Gosei Kawanishi |
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| Institution: | Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Ishibashi-machi, Shimotsuga-gun, Tochigi 329-05, Japan |
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| Abstract: | The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation. |
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| Keywords: | Monascus pilosus restriction-enzyme-mediated integration (REMI) aurintricarboxylic acid (ATA) |
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