Occurrence of a Tetrodotoxin-like Compound as a Minor Toxin in the Puffer Fish,Fugu flavidus |
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| Authors: | Hakaru Inaoka Kazuo Shiomi Hideaki Yamanaka Takeaki Kikuchi Tamao Noguchi Kanehisa Hashimoto |
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| Affiliation: | 1. Laboratory of Food Hygienic Chemistry, Tokyo University of Fisheries, Minato-ku, Tokyo 108, Japan;2. Laboratory of Marine Biochemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | Toxins in ovaries and livers of the puffer fish, Fugu flavidus, were purified successively on activated charcoal, Bio-Gel P-2 and Bio-Rex 70. Besides tetrodotoxin (TTX), a minor toxin (toxin D) was newly detected in both tissues; toxin D accounted for 3% and 4% of the totl lethal potency in ovaries and livers, respectively. Although there were differences in the specific lethal activity, dose-survival time relation and signs in mice between toxin D and TTX, positive reactions to Weber reagent and 10% KOH, and a negative one to 1% H2O2 were observed for both toxins. Moreover, toxin D, like TTX, gave rise to the C9-base upon alkali-degradation. These results suggested that toxin D is a TTX-like compound having a quinazoline skeleton. |
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