Changes of Hexosamine and Acetyl Contents during Cultivation of Extracellular Galactosamine-containing Polymer from Aspergillus parasiticus |
| |
Authors: | Osamu Hayashi Haruki Yamada Toshio Miyazaki |
| |
Institution: | Department of Microbial Chemistry, Tokyo College of Pharmacy, Hachioji, Tokyo 192-03 |
| |
Abstract: | The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated α and β anomeric forms of N-acetylchitooligosaccharides, and obtain the following advantages of this HPLC method.1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing α anomer formation from the retaining mechanism showing β anomer formation.2. We can also estimate the cleavage patterns of N-acetylchitooligosaccharides by chitinolytic enzymes by using natural substrates. |
| |
Keywords: | chitinase lysozyme chitinolytic enzyme N-acetylchitooligosaccharide |
|