Alcohol Dehydrogenases from Strawberry Seeds |
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Authors: | Ichiji Yamashita Kyuei Iino Seiji Yoshikawa |
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Institution: | National Food Research Institute, Ministry of Agriculture and Forestry, Shiohama 1-4-12, Koto-ku, Tokyo |
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Abstract: | Two alcohol dehydrogenases (alcohol: NAD oxidoreductase, EC 1.1.1.1 and alcohol: NADP oxidoreductase, EC 1.1.1.2) were partially purified from extracts of strawberry seeds by conventional methods. Some of physical, chemical and kinetic properties of the enzymes are described. On the basis of gel filtration, the molecular weights were estimated to be approximately 78,000 for NAD-dependent enzyme and 82,000 for NADP-dependent enzyme. Thiol-reacting compounds inhibited both enzymes. NAD-dependent alcohol dehydrogenase reacted only with aliphatic alcohols and aldehydes, while aromatic and terpene alcohols and aldehydes were the better substrates for NADP-dependent alcohol dehydrogenase than aliphatic alcohols and aldehydes. |
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Keywords: | vacuolar ATPase NtpK complementation Enterococcus hirae |
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