Intracellular Distributions of Enzymes and Intermediates Involved in Biosynthesis of Capsaicin and Its Analogues in Capsicum Fruits |
| |
| Authors: | Hideshi Fujiwake Tetsuya Suzuki Kazuo Iwai |
| |
| Affiliation: | 1. Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan;2. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan |
| |
| Abstract: | Phenylalanine ammonia-lyase, trans-cinnamate 4-monooxygenase, and capsaicinoid synthetase [Agric. Biol. Chem., 44, 2907 (1980)] activities were investigated in the subcellular fractions from protoplasts of placenta of Capsicum fruits. The subcellular distribution of intermediates of the capsaicinoid biosynthesis, trans-cinnamic acid and trans-p-coumaric acid, and capsaicinoid were also investigated. The activity of trans-cinnamate 4-monooxygenase and capsaicinoid synthetase was in the vacuole fraction. While the activity of phenylalanine ammonia-lyase was in the cytosol fraction. After feeding l-[U-14C]phenylalanine to the protoplast, the newly synthesized trans-p-coumaric acid and capsaicinoid were found in the vacuole fraction, while trans-cinnamic acid was not in the vacuole fraction. The possible role of the vacuole on the biosynthesis of capsaicinoid is also discussed. |
| |
| Keywords: | |
|
|
