Estimation of Parameters in Plasma Albumin Turnover by a Modified Double Isotope Method |
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| Authors: | Ryuhei Funabiki Nobuhiro Horisaka Atutane Ohta |
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| Institution: | Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University, Fuchu-shi, Tokyo 183, Japan |
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| Abstract: | Zygosaccharomyces rouxii contains at least two copies of the glyceraldehyde-3-phosphate dehydrogenase gene (GAP-DH). We cloned one of them, which is situated on a 9.5 kb Hindlll fragment, after detecting a sequence which is hybridizable with the GAP-DH gene of Saccharomyces cerevisiae. The amino acid sequence of the GAP-DH gene of Z. roxii deduced from its nucleotide sequence was compared with that of the GAP-DH genes of S. cerevisiae. The Z. rouxii enzyme is longer than the S. cerevisiae enzyme by one amino acid. 65, 66 or 71 amino acid changes were detected, depending on which GAP-DH gene of S. cerevisiae was compared with that of the Z. rouxii enzyme. The codon usage in the coding region of the GAP-DH gene of Z. rouxii is biased, as found in the case of the S.cerevisiae enzyme. |
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