X-Ray Crystal Structure of Pyrenocine A,a Phytotoxin from Pyrenochaeta terrestris |
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| Authors: | Hiroji Sato Keiichi Konoma Sadao Sakamura Akio Furusaki Takeshi Matsumoto Takao Matsuzaki |
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| Institution: | 1. Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan;2. Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060, Japan;3. Central Research Laboratories, Mitsubishi Chemical Industries, Midori-ku, Yokohama 227, Japan |
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| Abstract: | The milk fat globule membrane (MFGM) enclosing fat droplets in bovine milk was isolated, and its effects on hydrolysis of milk fat by lipases were investigated by using a gum arabic-stabilized milk fat emulsion as substrate. The addition of isolated MFGM to the reaction mixture markedly inhibited hydrolysis by pancreatic and microbial (Rhizopus delemer) lipases. The inhibition was completely lost on tryptic digestion of MFGM, suggesting that the protein moiety of MFGM played a role in the inhibition. Soluble glycoprotein (SGP) which was isolated from delipidated MFGM produced marked inhibitory activity. The inhibition by SGP was dependent on substrate concentration, suggesting that the inhibition was at least partly due to coverage and blockage of the substrate surface by SGP. |
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