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A Medium for the Isolation of Capsular Bacteria from Kefir Grains
Authors:Takahiro Toba  Susumu Abe  Keizo Arihara  Susumu Adachi
Institution:Laboratory of Animal Products Technology, College of Agriculture, Tohoku University, Tsutsumidori-Amamiyacho 1–1, Sendai 980, Japan
Abstract:Secondary alcohols (C3 to C10) were oxidized to the corresponding methylketones by resting mycelia of Scedosporium sp. A-4 grown on propane, but 3-pentanol and 3-hexanol were not oxidized. The oxidation of 2-propanol to acetone was inhibited by pyrazole, potassium cyanide, sodium azide and Hg2 +. Alcohol dehydrogenase activity was found in the cell-free soluble fraction and this activity requires a cofactor, specifically NAD+. The oxidation of both 1-propanol and 2-propanol may be catalyzed by the same alcohol dehydrogenase.
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