Studies on the Biosynthesis of Fosfomycin. Conversion of 2-Hydroxyethylphosphonic Acid and 2-Aminoethylphosphonic Acid to Fosfomycin |
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| Authors: | Satoshi Imai Haruo Seto Hiroshi Ogawa Atsuyuki Satoh Noboru Otake |
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| Institution: | 1. Pharmaceutical Development Laboratories, Meiji Seika Kaisha Ltd.,Saiwai-ku, Kawasaki 210, Japan;2. Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | Two types of 130 kDa insecticidal protein genes isolated from large plasmid DNAs of Bacillus thuringiensis var. israelensis HDS22 were cloned in an Escherichia coli plasmid vector. Analysis of the nucleotide sequences revealed that the two genes encoded a 127,500-dalton protein (ISRH3) consisting of 1,135 amino acids and a 134,400-dalton protein (ISRH4) consisting of 1,180 amino acids. The two insecticidal proteins were identical in a region of the C-terminal 467 amino acids. |
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