Increased Activity of Liver Xanthine Oxidase in Rats from Increasing Dietary Calcium |
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| Authors: | Hiroshi Sakahara Akira Satoh Norihisa Kato |
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| Affiliation: | Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, 724, Japan |
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| Abstract: | A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence. |
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| Keywords: | Arabidopsis binary vector selection marker tunicamycin |
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