Occurrence of Bacteriochlorophyll a in a Strain of an Aerobic Heterotrophic Bacterium |
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| Authors: | Keiji Harashima Tsuneo Shiba Tetsuya Totsuka Usio Simidu Nobuo Taga |
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| Affiliation: | 1. Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku and Nakano-ku, Tokyo;2. Ocean Research Institute, The University of Tokyo, Bunkyo-ku and Nakano-ku, Tokyo |
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| Abstract: | We developed a practical preparation procedure for K-252a by methylating K-252b on an industrial scale. The water-insoluble K-252a, which was present in the cell mass, was converted to the water-soluble K-252b Na salt in an alkaline solution. The obtained K-252b was methylated with dimethylsulfate in the presence of potassium carbonate in dimethylacetamide. We have already used this method to manufacture 90 kg of K-252b from the fermentation broth, and regenerated 65 kg of K-252a from K-252b. |
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| Keywords: | K-252a purification methylation |
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