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The Constituents of the Essential Oil from Litsea japonica (Thunb.) Juss., FRUIT
Authors:Hiromichi Nil  Mitsuo Iwakiri  Takashi Kubota
Institution:1. Ibaraki Research Laboratory of Nagaoka Perfumery Co., Ltd., 1-3-30, Itsukaichi, Ibaraki-shi567;2. Faculty of Medicine, Kinki University, 380, Nishiyama, Sayama-cho, Osaka589
Abstract:Tetragenococcus halophila D10 decarboxylates aspartate to alanine, but T. halophila D10 derivatives generated by a curing treatment could not (Asd- derivatives). We observed by electrophoresis three plasmid bands in T. halophila D10; all Asd- derivatives lost the largest of these bands. This plasmid, pD1, has two SalI sites. We cloned and sequenced the 10 kb SalI fragment. The DNA sequence suggests that this fragment contains the aspartate decarboxylating trait.
Keywords:Tetragenococcus halophila  plasmid  aspartate decarboxylation  soy sauce  curing
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