大鼠发育过程中肝、肺r-GT酶活性和定位

用生物化学和组织化学方法研究正常发育中大鼠肝、肺r-GT活性和定位。结果表明:肝r-GT活性自胚龄17天开始升高,21天达高峰,出生第一天明显降低,第六天降至接近成年低水平。在胚胎期肝r-GT主要位于肝细胞内,出生后则主要位于胆小管。该结果提示胚胎期肝r-GT主要参与肝细胞膜上氨基酸的转运。出生后可能主要参与解毒功能,大鼠肺r-GT活性随发育逐步升高,主要分布于肺支气管上皮细胞。提示肺r-GT可能参与解毒功能。

r-GT ENZYME ACTIVITY AND LOCATION OF LIVER AND LUNG DURING DEVELOPMENT OF RAT

The activity and location of r-GT in rat liver and lung were studied during normal development with biochemical and histochemical methods.It was found that the activity of r-GT in rat liver began to rise from 17-days-old-fetus and reached a high peak at 21-days-old-fetus. At 1 st day after birth, it decreased markedly, 6 th day after birth, it decreased to a low level of adult value. In fctal poriod, r-GT is mainly located in liver cells, but after birth, it is located mainly in the bile canaliculi.These results seem to indicate that fetal liver r-GT takes part in the transfer of arnino acids in cellular membrencc of the liver, After birth, it probably takes part in detoxination.The activity of lung r-GT increases gradually with development. Lung r-GT in adult rats is located in the epithelial cells of branchus.This result seems to indicate that the lung r-GT takes part in detoxination.