Sequence of a cellulase gene of Cellulomonas uda CB4 |
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| Institution: | 1. Division of Nephrology, Department of Medicine, Albany Stratton VA Medical Center, Albany, NY, USA;2. Division of Nephrology and Hypertension, Department of Medicine, University of Miami, Miami, FL, USA;3. Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ, USA;1. Consejo Nacional de Ciencia y Tecnología, Mexico;2. Universidad de Colima, Centro Universitario de Investigaciones Biomédicas, Mexico;3. Universidad de Colima, Facultad de Ingeniería Civil, Mexico;4. Instituto Mexicano del Seguro Social, Departamento de Epidemiología, Unidad de Medicina Familiar No. 19, Mexico;5. Universidad de Colima, Facultad de Medicina, Mexico;1. Department of Nephrology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 7708503, Japan;2. Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA;3. Department of General Medicine, Kawasaki Medical School, Kurashiki, 7010192, Japan;4. Department of Pharmacology, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 7708503, Japan;5. Department of Medical Technology, Tokushima University Hospital, Tokushima, 7708503, Japan;6. Department of Community Medicine for Diabetes and Metabolic Disorders, Tokushima University Graduate School of Biomedical Sciences, Tokushima, 7708503, Japan;7. Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, 7010192, Japan;1. Biotechnology Center (BIOTEC) of the Technische Universitat Dresden (TUD), Dresden, Germany;2. Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany;1. Department of Pathology & Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90024, USA;2. Bioinformatics Interdepartmental Program, University of California, Los Angeles, Los Angeles, CA 90024, USA;3. Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90024, USA;4. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA;5. Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA;6. Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA;7. Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90024, USA;1. International Renal Research Institute of Vicenza, San Bortolo Hospital, Vicenza, Italy;2. Department of Nephrology, Dialysis and Transplantation, San Bortolo Hospital, Vicenza, Italy;3. Department of Internal Medicine II, Division of Pulmonology, Nephrology and Critical Care Medicine, University Clinic Giessen and Marburg, Giessen, Germany;4. Department of Cardiac Surgery, San Bortolo Hospital, Vicenza, Italy;5. Department of Clinical Chemistry and Hematology Laboratory, San Bortolo Hospital, Vicenza, Italy;6. Department of Intensive Care, Austin Hospital, Melbourne, Victoria, Australia;7. Australian and New Zealand Intensive Care Research Centre, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia;8. Department of Medicine, University of Virginia Health System, Charlottesville, Virginia;9. Center for Critical Care Nephrology, CRISMA, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania |
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| Abstract: | The complete nucleotide sequence of the endoglucanase (CMCase) gene and its flanking regions of Cellulomonas uda CB4, a hyper producer of cellulase, was determined. A 1077 bp open reading frame for endoglucanase gene was observed. The endoglucanase secreted in E. coli was purified to homogeneous preparation and the amino acid sequence of the N-terminal region of the enzyme was determined to be Gln-Thr-Ala-Trp-Glu-Arg-Tyr-Lys-Ala-Arg-Phe-Met-Met-Pro-, suggesting the processing at Ala23 of pre-endoglucanase. The signal sequence consisted of 23 amino acids, of which one was Arg in the region near the N-terminus and 14 were hydrophobic amino acid residues having hydropathy indices greater than 1. |
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