Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose |
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| Institution: | 1. Universitat de Barcelona, Facultat de Biologia, Departament de Biologia Animal. Avda. Diagonal 645, 08028-Barcelona, Spain;2. Universitat de València, Facultat de Ciències Biològiques, Departament de Zoologia. Campus de Burjassot-Paterna, Dr. Moliner 50, E-46100 Burjassot (València), Spain;3. College of Forest and Biotechnology, Zhejiang Agricultural and Forestry University, Linan 311300, China;4. Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | A procedure is described for the reproducible establishment of rice cell suspension cultures from callus of embryo origin. Protoplasts were readily isolated from cell suspension of four rice cultivars, including japonica and indica types, when maintained in an amino acid-based culture medium. Sustained protoplast division from two japonica genotypes has been obtained in agarose solidified culture medium. An increase in the agarose concentration from 0.6% to 1.2% (w/v) produced a marked improvement in protoplast survival, division and plating efficiency. Protoplast division and plating efficiency frequencies of up to 26% and 0.5%, respectively, were obtained at the higher agarose level. The protoplast-derived calli were similar in appearance to explant-derived morphogenic callus and produced distinct embryo-like structures. |
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