Rapid and efficient regeneration of plants from explants of Arabidopsis thaliana |
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| Affiliation: | 1. Genetics and Plant Breeding Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, U.P., India;2. Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, U.P., India;3. Chemical Sciences Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, U.P., India;1. Comisión Chilena de Energía Nuclear, Departamento de Aplicaciones Nucleares, Laboratorio de Radiobiología Celular y Molecular. Nueva Bilbao 12501, Las Condes, Santiago, Chile;2. Universidad de Santiago de Chile, Centro de Biotecnología Acuícola, Laboratorio de Virología,Av. Bernardo O’Higgins 3303, Estación Central, Santiago, Chile;3. Universidad San Sebastián, Facultad de Ciencias, Lota 2465, Providencia, Santiago, Chile;1. Department of Biological Sciences, Boise State University, 1910 University Drive, Boise, ID 83725-1515, USA;2. University of Alabama, Department of Biological Sciences, Box 870345, Tuscaloosa, AL 35487, USA;1. Department of Chemistry, University of Bari “Aldo Moro”, via Orabona 4, 70126 Bari, Italy;2. Jaber Innovation s.r.l., via Calcutta 8, 00144 Rome, Italy;3. Department of Health Sciences, University of Piemonte Orientale “UPO”, via Solaroli 17, 28100 Novara, Italy;4. School of Engineering, Newcastle University, NE1 7RU Newcastle upon Tyne, UK;5. INSTM, Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, via Giuseppe Giusti 9, 50121 Firenze, Italy;6. Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, via Orabona 4, 70126 Bari, Italy;7. Department of Veterinary Medicine, University of Bari “Aldo Moro”, Str. Prov. Casamassima Km 3, 70010 Valenzano, Italy |
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| Abstract: | A procedure is given for the regeneration in vitro of a previously difficult to regenerate plant, Arabidopsis thaliana (L.) Heynh. Leaf explants of Arabidopsis that are given a short exposure to a callus-inducing medium prior to incubation on a shoot-inducing medium exhibit high survivability and rapidly produce shoots. Some media combinations allow shoots to form on 100% of the explants and the majority of these explants form multiple shoots. The shoots are normal in appearance and most of them can be induced to form functional roots and retain fertility. This regeneration scheme should be of use in the development of an Arabidopsis transformation procedure and may also be applicable to other recalcitrant plant species. |
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