Localization of partially methylated flavonol glucosides in Chrysosplenium americanum. I. Preparation and some properties of a trimethyl flavonol glucoside antibody |
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| Affiliation: | 1. Research Group of Physical-Chemistry of Condensed Matter, Department of Chemistry, Faculty of Sciences, Moulay Ismail University, Zitoune, 11201 Meknes, Morocco;2. Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, Zitoune, 11201 Meknes, Morocco;3. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy;4. Laboratory of Plant Biotechnologies and Molecular Biology, Department of Biology, Faculty of Sciences, Moulay Ismail University, Zitoune, 11201 Meknes, Morocco;5. Department of Food Science, College of Agriculture, University of Basrah, 61004 Basrah, Iraq;6. Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy;7. Department of Sciences and Technologies for Human and Environment, University Campus Bio, Medico of Rome, 00128 Rome, Italy;8. Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy;1. Department of Food Technology, Institute of Food Science and Nutrition, Faculty of Chemical and Food Technology of the Slovak University of Technology in Bratislava, Radlinského 9, Bratislava, 812 37, Slovak Republic;2. Department of Wood, Pulp, and Paper, Institute of Natural and Synthetic Polymers, Faculty of Chemical and Food Technology of the Slovak University of Technology in Bratislava, Radlinského 9, Bratislava, 812 37, Slovak Republic;1. Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, University of Helsinki, P.O. Box 56, FIN-00014, Finland;2. Faculty of Science and Forestry, Department of Environmental and Biological Sciences, University of Eastern Finland, 80101 Joensuu, Finland;3. Viikki Tropical Resources Institute (VITRI), Viikki Campus, University of Helsinki, FIN-00014, Finland;4. Department of Forest Products and Industries, Shambat Campus, University of Khartoum, SUD-13314, Sudan;5. Department of Forest Management, Shambat Campus, University of Khartoum, SUD-13314, Sudan;1. Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Egypt;2. Alexandria Main University Hospital, Alexandria, Egypt;3. Center of Excellence for Drug Preclinical studies (CE-DPS), Pharmaceutical and Fermentation Industry Development Center, City of Scientific Research & Technological Applications, New Borg El Arab, Alexandria, Egypt;4. Bio-screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt;5. Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt |
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| Abstract: | 5,2′,5′-Trihydroxy-3,7,4′-trimethoxyflavone-2′-O-glucoside, a major flavonoid constituent of Chrysosplenium americanum Schwein. ex Hooker was conjugated to bovine serum albumin (BSA) by the diazo reaction in good yield and with a molar ratio of 11.5:1. Antibody raised against the latter conjugate had a titer value of 1:1600 and was found to be specific for the 2′-O-glucosides of tri- and tetramethoxyflavones. Some cross reactivity (about 55%) was observed against the pentamethoxyflavone-5′-O-glucoside; but almost none with the parent hydroxyflavone, quercetin, or any of its partially methylated (3,7,4′-tri- or 3,7,3′,4′-tetramethyl-) derivatives. The specificity of antibodies raised against the 2′-O-glucosides of Chrysosplenium makes them useful for the intracellular localization of these natural constituents. |
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