Auxin binding activities in monocotyledonous and dicotyledonous tissue |
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| Affiliation: | 1. Sustainability Research Centre, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, Queensland 4556, Australia;2. Department of International Environmental and Agriculture Science, Tokyo University of Agriculture and Technology, Saiwai 3-5-8, Fuchu, Tokyo 1585809, Japan;3. Universidad Nacional de Colombia, Sede Medellín, Facultad de Minas, Office M2-312, Carrera 80 No 65-223, Núcleo Robledo, Medellín, Colombia |
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| Abstract: | A screening study was carried out in order to investigate the binding of NAA to subcellular fractions from various dark grown monocotyledonous and dicotyledonous tissues known to be physiologically responsive to NAA. Napthalene-1-acetic acid (NAA) binding results revealed differences between the monocots and dicots. Maize coleoptile tissue gave the highest concentration of saturable binding sites with a Kd-value of 1.56 μM and another in maize mesocotyl with a Kd-value of 0.83 M indicating a single class of binding site for NAA for both tissues. However, an inability to demonstrate saturable auxin binding in dicotyledonous tissues and maize root tissues was found. It is concluded that the results of this investigation do not negate the hypothesis that the high affinity saturable binding sites found in maize shoot tissues may function as auxin receptors. |
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