Localization of partially methylated flavonol glucosides in Chrysosplenium americanum. II. Immunofluorescence |
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| Affiliation: | 1. Department of Biology, Science Faculty, Selcuk University Campus, Konya, Turkey;2. Department of Environmental Engineering, Faculty of Engineering, University of Munzur, Tunceli, Turkey;3. Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius;4. Institute for Biological Research “Siniša Stanković” University of Belgrade, Belgrade, Serbia;5. Department of Analytical Chemistry, Pavol Jozef Šafárik University in Košice, Košice, Slovakia;6. Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary;7. Faculty of Sciences and Technology, Centre of Marine Sciences, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal |
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| Abstract: | Chrysosplenium americanum Schwein. ex Hooker (Saxifragaceae) accumulates a variety of partially methylated flavonol glucosides. Specific antibodies to tri- and tetramethylated flavonol-2′-O-glucosides, located using fluorescein isothiocyanate (FITC) goat antirabbit antibody, were used for the localization of the flavonol glucosides in leaf epidermis, cross sections and protoplasts. The results indicated that flavonoid accumulation occurred mainly in the walls of epidermal cells and, to a much lesser extent, in mesophyll cell walls. The weak fluorescence observed in the vacuoles of protoplasts suggested a minor role of this compartment in the accumulation process. The significance of flavonoid deposition within epidermal cell walls is discussed in relation to the lipophilic nature of these compounds and their possible role in the physiology of the plant. |
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