Detection of lectin-related sequences in the genome of Sesbania rostrata |
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| Affiliation: | 1. Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic;2. Department of Small Animal Science, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic;3. Departamento de Tecnología Agroalimentaria, Universidad Miguel Hernández de Elche, Orihuela, Spain;4. Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic;5. Research Centre AgroBioTech, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic;6. Institute of Farm Animals, Animal Production Research Centre Nitra, Luzianky, Slovak Republic;7. Department of Animal Physiology, Institute of Biology, Faculty of Exact and Natural Sciences, Pedagogical University of Cracow, Cracow, Poland |
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| Abstract: | The extensive homologies between the amino-acid sequences of lectins extracted from the seeds of Leguminous plants have been confirmed by using cDNAs corresponding to the lectin genes from Pea, French bean, and Soybean. These cDNAs, used as probes, were hybridized with restriction fragments of genomic DNA from Pea. Good cross-hybridizations were observed. This strategy was extended to another plant: Sesbania rostrata, a tropical legume for which the presence of lectins has not been reported yet. Several genomic fragments hybridize with the lectin cDNA probes, indicating the existence of lectin-related sequences. |
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