Cell cycle parameters and accumulation of metaphase cells in maize suspension cultures |
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| Affiliation: | 1. Department of Medical Sciences, Mercer University School of Medicine, Savannah, GA 31404, USA;2. SynPloid Biotek, LLC, Savannah, GA 31404, USA;1. Instituto Tecnológico Metropolitano – ITM, Calle 73 No. 76A-354, Medellín, Colombia;2. Universidad Nacional de Colombia Sede Medellín, Calle 59A 63-20, Medellín, Colombia;1. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;2. College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;3. Developmental Therapeutics Branch and Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892-4255, United States;1. C.D. Technologies Ltd., Mivza Yoav 16, Beer Sheba, Israel;2. Department of Science, Technology and Society, Bar Ilan University, Ramat Gan 5290002, Israel |
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| Abstract: | Cell cycle parameters of maize (Zea maysL cv Black Mexican Sweet) suspension cultures and root meristem cells were determined by pulse labelling with [3H]thymidine ([3H]TdR). Total cell cycle time for the suspension cultures was 27 h; 3 h in G1, 14 h in S, 6 h in G2, 2.2 h in prophase, 1 h in metaphase, 0.1 h in anaphase, and 0.7 h in telophase. Cell cycle durations in root meristem cells of Black Mexican Sweet (BMS) corn with and without B chromosomes in vivo were 20.0 h and 18.3h, respectively. Chemical and physical methods were used successfully to accumulate mitoses in the suspension cultures; compared to the untreated control, the mitotic index of the treated cultures was increased from 4 to 23% and the frequency of metaphase cells increased dramatically from 3 to 19%. |
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