The effects of extracellular calmodulin on initiation of Hippeastrum rutilum pollen germination and tube growth |
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Authors: | Ligeng Ma Daye Sun |
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Institution: | (1) Laboratory of Molecular Cell Biology, Hebei Normal University, Shijiazhuang, Hebei Province (050016), Peoples Republic of China, CN |
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Abstract: | The effects of anti-calmodulin (CaM) serum, the CaM antagonist W7-agarose, the Ca2+ chelator ethyleneglycol-bis-(β-aminoethyl)-N,N,N′,N′-tetraacetic acid (EGTA) and exogenous pure CaM on pollen germination
and tube growth of Hippeastrum rutilum Herb were studied. Pollen germination and tube growth were inhibited or completely stopped by anti-CaM serum in a dose-dependent
manner, while the same amount of preimmune serum had no effect on either process. Pollen germination and tube growth were
also inhibited or completely stopped by the CaM antagonist W7-agarose and the Ca2+ chelator EGTA. The addition of exogenous pure CaM enhanced pollen germination and tube growth, whereas the same amount of
bovine serum albumin had no effect. The inhibitory effects caused by anti-CaM serum, W7-agarose and EGTA-washing could be
reversed completely by the addition of exogenous pure CaM. These results indicate that extracellular CaM initiates pollen
germination and tube growth, whereas exogenous CaM enhances the above processes, and may provide a novel view for understanding
the control of pollen germination and tube growth.
Received: 12 December 1996 / Accepted: 15 January 1997 |
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Keywords: | Key words: Extracellular calmodulin Hippeastrum (pollen) Pollen germination Pollen tube growth |
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