| 褐藻裙带菜中PSII复合物及其亚复合物的分离和特性研究 |
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| 引用本文： | 陈敏,邢克克,李爱芬,周百成. 褐藻裙带菜中PSII复合物及其亚复合物的分离和特性研究[J]. 植物生理学通讯, 2006, 0(5) |
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| 作者姓名： | 陈敏 邢克克 李爱芬 周百成 |
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| 作者单位： | 山东烟台大学生物化学系 山东烟台264005(陈敏)，暨南大学水生生物研究中心 广州510632(邢克克，李爱芬)，中国科学院海洋研究所 山东青岛266071(周百成) |
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| 摘 要： | 用不连续梯度蔗糖密度超离心,从经TritonX-100增溶的褐藻裙带菜类囊体膜中分离到3种色素蛋白复合物条带,分别是捕光复合物、具有光氧化活性的PSII复合物颗粒(区带II)以及PSI(区带III)。PSII颗粒经毛地黄皂苷增溶后,再次超离心分离得到3条PSII的亚复合物条带。吸收和荧光激发谱显示其中的区带II-1为墨角藻黄素-Chla/c-蛋白复合物,区带II-2为Chla/c-蛋白复合物,两者都只含20kDa多肽;而鲜绿色的区带II-3为不含捕光复合物的活性PSII核心。
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| 关 键 词： | 裙带菜 蔗糖密度梯度离心 PSII PSII核心 捕光复合物 多肽 |
Study on Isolation and Characterization of PSII Pigment-protein Complex and Subcomplexes from a Brown Alga [Undaria pinnatifida (Harvey) Suringar] |
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| CHEN Min,,XING Ke-Ke,LI Ai-Fen,ZHOU Bai-Cheng. Study on Isolation and Characterization of PSII Pigment-protein Complex and Subcomplexes from a Brown Alga [Undaria pinnatifida (Harvey) Suringar][J]. Plant Physiology Communications, 2006, 0(5) |
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| Authors: | CHEN Min XING Ke-Ke LI Ai-Fen ZHOU Bai-Cheng |
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| Affiliation: | CHEN Min1,*,XING Ke-Ke2,LI Ai-Fen2,ZHOU Bai-Cheng3 1Department of Biochemistry,Yantai University,Yantai,Shandong 264005,China,2Research Center of Hydrobiology,Jinan University,Guangzhou 510632,China,3Institute of Oceanology,Chinese Academy of Sciences,Qingdao,Shandong 266071,China |
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| Abstract: | Three pigment-protein complexes which were light-harvesting complex and active PSII (zone II) and PSI (zone III) particles were isolated from a brown alga Undaria pinnatifida (Harvey) Suringar by Triton X-100 sucrose gradient centrifugation.The three subcomplexes were separated by further density gradient after digitonin solubilization of PSII particle.Zone II-1 was a fucoxanthin-Chl a/c-protein complex,and zone II-2 was a Chl a/c-protein complex according to their absorption and fluorescence spectra.They all presented a major peptide component at 20 kDa.The fresh green fraction zone II-3 was a active PSII core complex depleting light-harvesting complexes. |
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| Keywords: | Undaria pinnatifida (Harvey) Suringar sucrose gradient centrifugation PSII PSII core light-harvesting complex peptide |
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