Expression profiling of <Emphasis Type="Italic">Streptomyces peucetius</Emphasis> metabolic genes using DNA microarray analysis |
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Authors: | Joon-Ryeol Yang Eunjung Song Byung-Gee Kim Eung-Soo Kim Jae-Kyung Sohng Min-Kyu Oh |
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Institution: | (1) Department of Chemical and Biological Engineering, Korea University, Seoul, 136-713, Korea;(2) School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Korea;(3) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(4) Department of Pharmaceutical Engineering, SunMoon University, Asan, 336-708, Korea |
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Abstract: | Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as
well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation.
To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes
involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two
different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main
precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle
were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR. |
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Keywords: | Streptomyces peucetius DNA microarray doxorubicin metabolic gene |
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