| 454测序法在环境微生物生态研究中的应用 |
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| 引用本文： | 徐晓宇,刘和.454测序法在环境微生物生态研究中的应用[J].生物技术通报,2010(1). |
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| 作者姓名： | 徐晓宇 刘和 |
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| 作者单位： | 1. 江南大学医药学院,无锡,214122
2. 江南大学环境与土木工程学院,无锡,214122 |
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| 基金项目： | 江苏省科技支撑计划社会发展项目 |
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| 摘 要： | 传统的Sanger测序技术虽已成熟,但其速度和成本的限制满足不了大规模测序的要求。第二代高通量测序技术结合了乳胶微粒和皮升级反应的454焦磷酸测序法,作为一种高通量测序技术,具有分析结果准确、高速、高灵敏度和高自动化的特点。对454测序法的技术原理和操作步骤进行了介绍,对近年来运用该方法在环境微生物生态研究领域的进展进行了综述。
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| 关 键 词： | 454测序 环境微生物生态 宏基因组 |
Application of 454 Sequencing in Environmental Microbial Ecology |
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| Xu Xiaoyu,Liu He.Application of 454 Sequencing in Environmental Microbial Ecology[J].Biotechnology Bulletin,2010(1). |
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| Authors: | Xu Xiaoyu Liu He |
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| Institution: | Xu Xiaoyu1 Liu He2(1School of Medicine , Pharmaceutical,Wuxi 214122,2School of Environment , Civil Engineering Jiangnan University,Wuxi 214122) |
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| Abstract: | There has been a desire to increase the throughput of DNA sequencing ever since Sanger sequencing by chain termination or fragmentation techniques coupled with electrophoretic size separation.454 sequencing is the pyrosequencing which combined with microfluidics and picoliter reaction.In this article,the development of the 454 sequencing system was discussed,and the application of 454 sequencing in environmental microbial ecology was reviewed. |
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| Keywords: | 454 sequencing Environmental microbial ecology Metagenomics |
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