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Streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs |
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| Authors: | Rinkinen Minna L Koort Joanna M K Ouwehand Arthur C Westermarck Elias Björkroth K Johanna |
| Affiliation: | Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, P.O. Box 57, University of Helsinki, 00014 Helsinki, Finland;Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 57, University of Helsinki, 00014 Helsinki, Finland;Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland |
| Abstract: | Canine intestinal lactic acid bacterium (LAB) population in four fistulated dogs was cultured and enumerated using MRS agar. LAB levels ranging from 1.4x10(6) to 1.5x10(7) CFU ml(-1) were obtained in jejunal chyme. In the fecal samples 7.0x10(7) and 2.0x10(8) CFU g(-1) were detected. Thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16S and 23S rDNA restriction fragment length polymorphism patterns (ribotyping) and 16S rDNA sequence analysis. According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both feces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected. |
| Keywords: | Culturable canine intestinal lactic acid bacteria Jejunal chyme Streptococcus alactolyticus |
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