Tropic1808基因作用于PC12细胞的表达谱芯片分析

为探讨tropic1808基因作用的分子机制,采用高密度寡核苷酸芯片(Affymetrix芯片)对表达tropic1808基因和空载体的PC12细胞株进行转录水平分析.基于获得的基因表达信息,对UCSC、TRANSFAC、NCBI等公共数据库进行检索,观察表达tropic1808基因导致PC12细胞株的基因表达谱变化.在检测的15866个目标基因中,855个基因表达上调,80个有显著比较意义,涉及包括粘附因子、离子通道、信号转导、细胞代谢等基因成员.其中包括多个细胞粘附因子及与细胞分化、神经发生和突触发生的有关基因.推测Tropic1808基因过表达可诱导PC12细胞株中粘附因子基因水平上调及与细胞分化、神经发生和突触发生相关的基因表达上调.

Gene Expression Profiles in PC12: Alterations Induced by Tropic1808 Gene Over Expression

To survey the molecular alterations in a broader way, Affymetrix genechip was used to investigate the possible changes of mRNA level in PC12 cells over expression tropic1808 gene by comparison to control cells. Of the 15 866 examined genes and expressed sequence tags (ESTs), the expression of 855 genes were up-regulated, and 80 of them significantly increased by two folds or more. These genes were involved in a number of distinct families, including cadherins, ion channels, signal transduction molecules, metabolism and others.Increased mRNA levels of CRABPⅡ,Cdh1,Ascl2,and Lgals3 were further confirmed by RT_PCR. Our findings suggested that a number of cadherins were up-regulated and some genes concerning to cell differentiation, synaptogenesis and neurogenesis were also increased their expression in PC12 cells over-expressing tropic1808 gene.