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Implanted bone marrow-derived mesenchymal stem cells fail to metabolically stabilize or recover electromechanical function in infarcted hearts |
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| Authors: | Eun L Y Song H Choi E Lee T G Moon D W Hwang D Byun K H Sul J H Hwang K C |
| Institution: | a Yonsei University Graduate School of Medicine, Seoul 120-752, Republic of Korea b Division of Pediatric Cardiology, Myongji Hospital Cardiovascular Center, Kwandong University Health System, Goyang 412-270, Republic of Korea c Department of Biochemistry and Molecular Biology, Chosun University School of Medicine, Gwangju 501-759, Republic of Korea d Cardiovascular Research Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea e Center for Nanobio Convergence Research, Korea Research Institute of Standards and Science, Daejeon 305-600, Republic of Korea f Department of Nano Surface Science, University of Science and Technology, Daejeon 305-600, Republic of Korea g School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Engineering, POSTECH, Pohang 790-784, Republic of Korea h Division of Cardiology, Myongji Hospital Cardiovascular Center, Kwandong University Health System, Goyang 412-270, Republic of Korea i Division of Pediatric Cardiology, Severance Cardiovascular Hospital, Yonsei University Health System, Seoul 120-752, Republic of Korea j Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea |
| Abstract: | Mesenchymal stem cells (MSCs) have been used with success in several clinical applications for clinical treatment of ischemic hearts. However, the reported effects of MSC-based therapy on myocardial infarction (MI) are inconsistent. In particular, the preventive effects of MSC-based therapy on arrhythmic sudden death and metabolic disorders after infarction remain controversial. Here, we investigated the effects of MSCs on reverse remodeling in an infarcted myocardium, and found that MSC-therapy failed to achieve the complete regeneration of infarcted myocardium. Histological analyses showed that although infarct size and interstitial fibrosis induced by MI recovered significantly after MSC treatment, these improvements were marginal, indicating that a significant amount of damaged tissue was still present. Furthermore, transplanted MSCs had slight anti-apoptotic and anti-inflammatory effects in MSC-implanted regions and no significant improvements in cardiac function were observed, suggesting that naïve MSCs might not be the right cell type to treat myocardial infarction. Furthermore, small ion profiling using ToF-SIMS revealed that the metabolic stabilization provided by the MSCs implantation was not significant compared to the sham group. Together, these results indicate that pretreatment of MSCs is needed to enhance the benefits of MSCs, particularly when MSCs are used to treat arrhythmogenicity and metabolically stabilize infarcted myocardium. |
| Keywords: | MSCs mesenchymal stem cells MI myocardial infarction ToF-SIMS time-of-flight secondary ion mass spectroscopy |
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