Rapid detection of <Emphasis Type="Italic">Vibrio metschnikovii</Emphasis> in aquatic products by real-time PCR |
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Authors: | J Cao J Xu Q Zheng P Yan |
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Institution: | Liaoning Entry-Exit Inspection & Quarantine Bureau, Dalian 116001, China. cjj0909@163.com |
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Abstract: | PCR and SYBR Green I real-time PCR techniques were applied to the rapid detection of Vibrio metschnikovii by designing primers based on infC (initiation factor 3) gene sequence. The specificity, sensitivity, and practical applications of the methods have been also
analyzed. The methods showed high detecting specificity with no cross amplifications of other closely related and nonrelated
species; they provide a simple and rapid tool for V. metschnikovii detection with high sensitivity and specificity |
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