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Structure of ribosomal RNA gene and phytogeny ofNosema isolates in Korea |
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| Authors: | Jiyoung Choi Jonggill Kim Youngcheol Choi Sung-Hee Nam James Russell Wontae Kim Jan Wuyts Yeonho Je Gilsang Jeong |
| Affiliation: | 1. Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 441-853, Suwon, Republic of Korea 2. Department of Biology, University of California, 92521, Riverside, USA 3. Department of Plant Biology, Flander Interuniversity Institute for Biotechnology (VLB), Ghent University, Technologiepark 927, B9052, Ghent, Belgium 4. Department of Agricultural Biotechnology, Seoul National University, 151742, Seoul, Republic of Korea |
| Abstract: | The ribosomal RNA (rRNA) gene region of the fourNosema sp. isolates (C01, C02, C03 and C04) fromPieris rapae in Korea has been examined. Complete DNA sequence data (3779 bp) of The rRNA gene ofNosema sp. C01 are presented for the small subunit gene (SSU rRNA: 1236 bp), the internal transcribed spacer (ITS: 37 bp), and the large subunit gene (LSU rRNA 2506 bp). The secondary structures ofNosema sp. COI SSU and LSU rRNA genes are constructed and described. The SSU rRNA showed a hypervariable V4 region identified four additional stems including a pseudoknot. Phylogenetic analysis based on the SSU rRNA suggests that the four isolates belong to the ‘true’Nosema group. In contrast to theNosema/Vairimorpha clade, the members of the group are highly divergent. |
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