| 端粒酶反义cDNA对乳腺癌细胞损伤修复能力的影响 |
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| 引用本文： | 张晓伟,陈彦灵,童坦君.端粒酶反义cDNA对乳腺癌细胞损伤修复能力的影响[J].中国生物化学与分子生物学报,2001,17(3):401-405. |
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| 作者姓名： | 张晓伟 陈彦灵 童坦君 |
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| 作者单位： | 北京大学医学部生物化学与分子生物学系，北京 100083 |
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| 基金项目： | 国家自然科学基金重点项目(No.39930170)及国家重点基础研究发展规划(No.G2000057001)资助. |
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| 摘 要： | 利用反义核酸技术将端粒酶RNA的全长cDNA反向导入乳腺癌MCF 7细胞基因组中 .通过单细胞凝胶电泳实验发现 ,其DNA受H2 O2 损伤后的修复能力下降 .但端粒酶活性抑制为何引起其DNA损伤修复能力下降的原因尚待进一步研究 .
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| 关 键 词： | 端粒酶 反义核酸技术 MCF-7 损伤修复 |
| 收稿时间： | 2001-06-20 |
| 修稿时间： | 2000年9月14日 |
Effect of Antisense Telomerase cDNA on the DNA Repair Ability of Breast Cancer Cells |
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| ZHANG Xiao wei,CHEN Yan ling,TONG Tan jun.Effect of Antisense Telomerase cDNA on the DNA Repair Ability of Breast Cancer Cells[J].Chinese Journal of Biochemistry and Molecular Biology,2001,17(3):401-405. |
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| Authors: | ZHANG Xiao wei CHEN Yan ling TONG Tan jun |
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| Institution: | (Department of Biochemistry and Molecular Biology,Peking University Health Science Center,Beijing 100083,China |
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| Abstract: | By directing the synthesis of the telomeric repeats to the chromosome ends,telomerase prevented the decline of cellular proliferation with telomere abridging.Tumor cells,stem cells and some other cells showed high activities of telomerase.With antisense oligonucleotide technique,the whole cDNA of human telomerase RNA(hTR)was integrated inversely into human breast cancer cells MCF 7 in which antisense hTR RNA was expressed constantly.The results showed that the downregulation of telomerase activity significantly suppressed the DNA repair ability by single cell gel electrophoresis assay,though the mechanism was not clear yet. |
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| Keywords: | telomerase antisense ribonucleotide technique MCF 7 damage repair ability |
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