Biotechnological methods of <Emphasis Type="Italic">in vitro</Emphasis> propagation in willows (<Emphasis Type="Italic">Salix</Emphasis> spp.) |
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Authors: | Dagmar Skálová Božena Navrátilová Lenka Richterová Michal Knit Michal Sochor Radim J Vašut |
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Institution: | (1) Laboratory of General Botany, Institute of Experimental Biology, Umultowska 89, 61-614 Poznan, Poland;(2) Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland |
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Abstract: | Many populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are
thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments
of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green
young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination
of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were
cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium was the most efficient
one. In this case, only the microcalluses of S. viminalis were observed. We developed biotechnological procedures that can be useful in conserving fragmented populations of high-mountainous
willows. |
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