| 巴西橡胶树HbSIP2互作蛋白的筛选和鉴定 |
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| 引用本文： | 朱家红,徐靖,郭冬,李辉亮,王颖,彭世清. 巴西橡胶树HbSIP2互作蛋白的筛选和鉴定[J]. 基因组学与应用生物学, 2019, 38(2): 681-686 |
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| 作者姓名： | 朱家红 徐靖 郭冬 李辉亮 王颖 彭世清 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术研究所,海口,571101;海南省农业科学院粮食作物研究所,海南省农作物遗传育种重点实验室,海口,571100 |
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| 摘 要： | 可溶性无机焦磷酸酶在天然橡胶生物合成中具有重要的调控作用,HbSIP2是胶乳中关键的可溶性无机焦磷酸酶基因。为了深入了解HbSIP2调控橡胶生物合成的机理,本研究对HbSIP2互作蛋白进行了筛选和鉴定。结果表明:诱饵载体p GBKT7-Hb SIP2无自激活活性,且对酵母无毒性作用,可以用于酵母文库筛选。将诱饵载体与橡胶树胶乳cDNA文库进行杂交,初步筛选获得20个与HbSIP2互作的蛋白。进一步通过双分子荧光互补实验证实,Hb SIP2能够与橡胶延伸因子发生蛋白互作。本研究结果为HbSIP2调控橡胶生物合成的机理研究提供了重要的理论依据。
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| 关 键 词： | 巴西橡胶树 HbSIP2 蛋白互作 橡胶延伸因子 |
Screening and Identification of Proteins Interacting with HbSIP2 in Hevea brasiliensis |
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| Zhu Jiahong,XU Jing,Guo Dong,Li Huiliang,Wang Ying,Peng Shiqing. Screening and Identification of Proteins Interacting with HbSIP2 in Hevea brasiliensis[J]. Genomics and Applied Biology, 2019, 38(2): 681-686 |
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| Authors: | Zhu Jiahong XU Jing Guo Dong Li Huiliang Wang Ying Peng Shiqing |
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| Affiliation: | (Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Institute of Cereal Crops,Hainan Academy of Agricultural Sciences and Key Laboratory of Crop Genetics and Breeding of Hainan Province,Haikou,571100) |
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| Abstract: | Soluble inorganic pyrophosphatase plays an important role in natural rubber biosynthesis;HbSIP2 is a key soluble inorganic pyrophosphatase gene in latex. In order to understand the mechanism of HbSIP2 regulating rubber biosynthesis, proteins interacting with HbSIP2 were screened and identified. The results showed that bait plasmid pGBKT7-HbSIP2 had no self-activating activity and no cytotoxicity to the yeast cells, so it can be used to screen yeast library. The bait plasmid pGBKT7-Hb SIP2 was hybridized with latex cDNA library, and 20 proteins interacting with HbSIP2 were obtained. It was further confirmed by bimolecular fluorescence complementation analysis that HbSIP2 could interact with rubber elongation factor. This study might provide an important theoretical basis for further revealing the mechanism of Hb SIP2 regulating rubber biosynthesis. |
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| Keywords: | Heveab rasiliensis HbSIP2 Protein interaction Rubber elongation factor |
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